Hso-Chi Chaung
National Pingtung University of Science and Technology, Taiwan
Title: Co-culture of T subsets and antigen-presenting cells to form immune synapse
Biography
Biography: Hso-Chi Chaung
Abstract
The importance of the interactions of pathogens and the immune system has played a extremely important role in agricultural research for development of vaccinations. Porcine reproductive and respiratory syndrome (PRRSV) causes severe economic loss to the swine industry worldwide. The aim of this study is to establish a immune synapse system by co-culturing T subset/antigen-presenting cell as a fast screening system. In this study, blood samples were collected from 12 SPF pigs that were randomly separated into 3 groups for the control group and two different vaccinated immunizing PRRSV subunit vaccines (PRRSV-1 and PRRSV-2). Different T subsets by fluorescence-activated cell sorting were co-cultured with alveolar macrophage and the profiles of cytokine secretion and mRNA transcription levels of toll-like receptors were examined. The results showed that there were significant interaction differences in IL2, TLR3, TLR8, and TLR9 expressions between vaccinated groups and co-cultured cell systems. For instance, TLR8 expressions in CD4+CD25+ cells of PRRSV-2 (1.005±0.865) were lower than those in same cell system of controls (2.625±1.552). Interesting, the significantly lower IL-10 production were observed in animals vaccinated with PRRSV-2 (1.459± 0.396) as compared with those in control (4.155±1.494) or vaccinated with PRRSV-1 (4.781±1.135). These findings demonstrated that a porcine immune synapse system can be potentially used as screening the micro-environment factors as the adjuvant candidate or effective antigen epitopes. This system will be beneficial in reducing the use of experimental animals when studying the immune system whilst developing disease-resistance biomarkers or immune-modulatory adjuvants.